Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS
The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI-MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5-1000 ng/mL and 1-1000 ng/mL for curcumin-O-glucuronide with 85-115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.
Publication Source (Journal or Book title)
Journal of pharmaceutical and biomedical analysis
Liu, Y., Siard, M., Adams, A., Keowen, M. L., Miller, T. K., Garza, F., Andrews, F. M., & Seeram, N. P. (2018). Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS. Journal of pharmaceutical and biomedical analysis, 154, 31-39. https://doi.org/10.1016/j.jpba.2018.03.014