Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS

Yongqiang Liu, Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI, 02881, USA.
Melissa Siard, Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA.
Amanda Adams, Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA.
Michael L. Keowen, Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
Travis K. Miller, Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
Frank Garza, Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
Frank M. Andrews, Equine Health Studies Program, Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Skip Bertman Drive, 1843 Veterinary Teaching Hospital, Baton Rouge, LA, 70803, USA.
Navindra P. Seeram, Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI, 02881, USA. Electronic address: nseeram@uri.edu.

Document Type

Article

Publication Date

5-30-2018

Abstract

The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI-MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5-1000 ng/mL and 1-1000 ng/mL for curcumin-O-glucuronide with 85-115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.

Publication Source (Journal or Book title)

Journal of pharmaceutical and biomedical analysis

First Page

31

Last Page

39

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