Complete plastic nanofluidic devices for DNA analysis via direct imprinting with polymer stamps
Development of all polymer-based nanofluidic devices using replication technologies, which is a prerequisite for providing devices for a larger user base, is hampered by undesired substrate deformation associated with the replication of multi-scale structures. Therefore, most nanofluidic devices have been fabricated in glass-like substrates or in a polymer resist layer coated on a substrate. This letter presents a rapid, high fidelity direct imprinting process to build polymer nanofluidic devices in a single step. Undesired substrate deformation during imprinting was significantly reduced through the use of a polymer stamp made from a UV-curable resin. The integrity of the enclosed all polymer-based nanofluidic system was verified by a fluorescein filling experiment and translocation/stretching of λ-DNA molecules through the nanochannels. It was also found that the funnel-like design of the nanochannel inlet significantly improved the entrance of DNA molecules into nanochannels compared to an abrupt nanochannel/microfluidic network interface.
Publication Source (Journal or Book title)
Lab on a chip
Wu, J., Chantiwas, R., Amirsadeghi, A., Soper, S. A., & Park, S. (2011). Complete plastic nanofluidic devices for DNA analysis via direct imprinting with polymer stamps. Lab on a chip, 11 (17), 2984-9. https://doi.org/10.1039/c1lc20294d