Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging
Sushant P. Sahu, Department of Chemistry, University of Louisiana at Lafayette, Lafayette, LA 70504, USA.
Qianglin Liu, LSU AgCenter, School of Animal Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Alisha Prasad, Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Syed Mohammad Hasan, Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Qun Liu, Department of Computer Science, Louisiana State University, Baton Rouge, LA 70803, USA.
Maria Ximena Rodriguez, Department of Computer Science, Louisiana State University, Baton Rouge, LA 70803, USA.
Orna Mukhopadhyay, Baton Rouge Magnet High School, Baton Rouge, LA 70806, USA.
David Burk, Shared Instrumentation Facility and Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA.
Joseph Francis, Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Supratik Mukhopadhyay, Department of Computer Science, Louisiana State University, Baton Rouge, LA 70803, USA.
Xing Fu, LSU AgCenter, School of Animal Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Manas Ranjan Gartia, Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Abstract
We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and immunofluorescence imaging using fluorescently-labeled collagen antibodies. The post-mortem cardiac tissue imaging using SHG demonstrated a progressive increase in collagen deposition in the left ventricle (LV) post-MI. We were able to monitor structural morphology and LV remodeling parameters in terms of extent of LV dilation, stiffness and fiber dimensions in the infarcted myocardium.
