Identifier
etd-11102015-091749
Degree
Master of Science in Biological and Agricultural Engineering (MSBAE)
Department
Biological and Agricultural Engineering
Document Type
Thesis
Abstract
Nanoparticles (NPs) have recently been developed for application as drug carriers in different fields, including biomedical engineering. This study was focused on the use of a millifluidic chip for synthesis of polymeric poly (lactic co-glycolic acid) (PLGA) NPs and the understanding of PLGA NPs interaction and effect on red blood cells (RBCs). A method for NPs synthesis on a millifluidic chip was developed, exploiting the precise flow control offered by this device. PLGA NPs ranging from 230nm to 350nm in diameter were produced. Smaller NPs of lower polydispersity were synthesized at higher flow rates of the aqueous phase; increase in the ratio of aqueous phase to organic phase also decreased the size of the NPs prepared. The interaction of the PLGA NPs synthesized with the millifluidic chip with RBCs was visualized using a scanning electron microscope and quantified by flow cytometry. The effect of PLGA NPs on RBCs integrity was studied by a hemolysis assay. Results indicated that PLGA NPs adhered to the surface of red blood cells, but had no significant hemolytic effect at concentrations lower than 10mg/ml.
Date
2015
Document Availability at the Time of Submission
Secure the entire work for patent and/or proprietary purposes for a period of one year. Student has submitted appropriate documentation which states: During this period the copyright owner also agrees not to exercise her/his ownership rights, including public use in works, without prior authorization from LSU. At the end of the one year period, either we or LSU may request an automatic extension for one additional year. At the end of the one year secure period (or its extension, if such is requested), the work will be released for access worldwide.
Recommended Citation
Libi, Sumit, "Poly (Lactic Co-Glycolic) Acid Nanoparticles: Synthesis Using Millifluidic Chip and Interaction with Red Blood Cells" (2015). LSU Master's Theses. 808.
https://repository.lsu.edu/gradschool_theses/808
Committee Chair
Sabliov, Cristina
DOI
10.31390/gradschool_theses.808