Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein
Master of Science in Biological and Agricultural Engineering (MSBAE)
Biological and Agricultural Engineering
The published benefits of polymeric nanoparticles as a system for antioxidant delivery have encompassed topics of improved oral delivery, bioavailability, and modified release to name a few. Generally accepted as safe polymers Poly(lactic-coglycolic acid) and the mucoadhesive chitosan were selected to entrap alpha-tocopherol and lutein. This study proposed to assess the physical stability of PLGA and Chi/PLGA nanoparticles with entrapped bioactives in varying biological suspension medias ( water, pbs, hbss and cell media). Also, to assess the chemical stability of the entrapped antioxidants lutein and alpha-tocopherol within the same media as deemed relevant for in-vitro and and in-vivo studies. Of the tested media, physical stability was not media dependent for the PLGA(AO) or PLGA/Chi (AO) particles. Neither the size nor the PDI appeared significantly affected by the media it was re-suspended. Entrapped alphatocopherol remained chemically stable throughout the time suspended in the various biological mediums. While the lutein emulsion stability decreased over time, the NP(L)demonstrated improved stability. Also, the entrapped lutein stability was not dependent on the selected medium. Identifying any potential effects of suspension media on polymeric nanoparticles and the entrapped bioactive affords the opportunity to improve analysis of more robust or complex nanoparticle studies. Also, presented are considerations that should be taken prior to design of an experiment.
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Whaley, Meocha, "Physical Stability of Poly (lactic-co-glycolic acid) and PLGA/Chitosan Nanoparticles and Chemical Stability of Entrapped Alpha-Tocopherol and Lutein" (2014). LSU Master's Theses. 544.