Master of Science in Chemical Engineering (MSChE)
Genomic DNA is constantly in danger of being damaged by endogenous cellular processes and exogenous agents. Eukaryotes have mechanisms, collectively termed the DNA damage response, that detect and repair DNA damage. After a double-stranded break occurs, protein complexes are recruited to the break site to promote resection. Two main protein kinases involved in DNA repair are the Tel1 and Mec1 proteins. Recent studies have found that the Tel2 protein binds to both the Tel1 and Mec1 proteins and a point mutation of TEL2 on the 129th amino acid disrupts this interaction. Since both of the Tel1 and Mec1 proteins are involved in DNA repair, we believe disruption of this interaction might cause defects in the resection process. A quantitative real-time PCR assay was used to analyze single-stranded DNA content on the MAT locus at three specific sites distal to the break. It was observed that cells containing the point mutation S129N on the TEL2 gene resulted in a lower amount of single-stranded DNA content compared to wild-type cells. These findings show that Tel2 might play a role in the resection process.
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Trieu, Tommy Nhan, "Saccharomyces cerevisiae Tel1 & Tel2 role in DNA double-strand break repairs" (2015). LSU Master's Theses. 3434.