Doctor of Philosophy (PhD)
Engineering Science (Interdepartmental Program)
The development of porous materials useful as scaffolds for the sustained three-dimensional (3D) growth of human adipose-derived stem cells (hASC) is of particular interest to facilitate healing after musculoskeletal injuries. In this study, a composite porous material obtained by blending akermanite with poly-e-caprolactone (PCL) is proposed as novel alternative to bone tissue regeneration. The objectives of this study are (1) to characterize the akermanite:PCL scaffold properties; (2) to investigate the in vitro osteogenic potential of hASC loaded to optimal akermanite:PCL scaffolds; (3) to assess the metabolic activity and osteogenesis of hASC loaded to optimal akermanite:PCL scaffolds post-thawing using optimal cryopreservation protocol; and (4) to evaluate the behavior of optimal akermanite:PCL scaffolds in vivo using an immunodeficient murine model for ectopic bone formation. We hypothesized that (1) optimal akermanite:PCL blend has mechanical properties and biocompatibility suitable for tissue engineering applications; (2) hASC loaded to optimal akermanite:PCL scaffolds has higher expression of mature osteogenic marker in scaffolds cultured in osteogenic medium for 21 days; (3) PVP-serum free medium can be used to cryopreserve hASC loaded to optimal akermanite:PCL scaffolds; and (4) hASC preloaded to optimal akermanite:PCL scaffolds would produce meaningful bone-like tissue 8 weeks post-implantation. According to the results, 75:25 akermanite:PCL composite scaffolds displayed increased mechanical (1), biological and osteogenic properties (1-3). Moreover, hASC loaded to 75:25 akermanite:PCL scaffolds and frozen at 40ᵒC/min displayed metabolic activity and osteogenesis comparable to fresh control scaffolds (3). However, in vivo implantation of akermanite-base scaffolds (akermanite and akermanite:PCL) in nude mice, sudden death within the first 48 hours of this study (4). The acute toxicity observed in all animals assigned to the akermanite scaffolds was associated to a disturbance of the phosphorus homeostasis in vivo. Specifically, akermanite and akermanite:PCL scaffolds harvested 48 hours post-implantation had comparable levels of phosphorous in the composition, indicating acute phosphorous depletion from the serum. Accumulative evidences have suggested that akermanite is biocompatible and can enhance adhesion, proliferation and osteogenic phenotype maintenance of adult/osteoprogenitor stem cells both in vitro and in vivo. As a conclusion, further studies are needed to address the akermanite dose-dependent toxicity in murine models for akermanite-assisted bone regeneration.
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Zanetti, Andre S., "Characterization of Novel Akermanite:Poly-E-Caprolactone Scaffolds for Bone Tissue Engineering Aapplications Combined with Human Adipose-Derived Stem Cells" (2011). LSU Doctoral Dissertations. 2797.
Hayes, Danie J.