Doctor of Philosophy (PhD)
The visual determination of saccharides has been of interest for well over a century. Sugars are a relatively challenging class of compounds to analyze. They exhibit great structural similarity as well as transparency in the visible region. The sensing of specific saccharides could aid the monitoring of disease or industrial fermentation products. Herein I present new methods allowing for simple and rapid visual detection of sialic acid, fructose and a homologous series of prototypical neutral oligosaccharides, the linear maltodextrins, containing up to seven glucose residues. The new methods allow for selective color detection of fructose in a large excess of glucose and simultaneously determination of glucose. The fundamental problem of diminished optical detection of larger oligosaccharides is overcome using simple colorimetric agents. In addition, the first evidence that xanthenes form and serve as the active chromophores in resorcinarene solutions is presented. Meanwhile, the saccharide-induced optical signal transduction mechanism is investigated. A synthesis of resorcinarene derivatives, which have potential application in liposome stabilization, is also described.
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He, Ming, "Synthesis and study of new materials for the visual detection of biomolecules" (2002). LSU Doctoral Dissertations. 2124.
Robert M. Strongin