The synthesis of deuterated arylamine DNA adducts for use in the development of an isotope dilution LC/MS/MS method
Doctor of Philosophy (PhD)
Polycyclic aromatic amines (arylamines) are a class of chemical carcinogens that are prevalent in environmental and industrial settings. In order to study the mechanism of their toxicity a quantitative and qualitative detection method was developed to measure the C8-adenine adducts of benzidine and 2-aminofluorene in DNA samples. To do this a novel synthetic method using a palladium catalyst was developed to prepare authentic and deuterated arylamine adducts to serve as standards. These standards were then used to develop a high performance liquid chromatography, electrospray, tandem mass spectrometry, isotope dilution, detection method. To demonstrate the validity of this method two spike and recovery experiments were performed.
Document Availability at the Time of Submission
Release the entire work immediately for access worldwide.
Olsen, Philip Dmitri, "The synthesis of deuterated arylamine DNA adducts for use in the development of an isotope dilution LC/MS/MS method" (2001). LSU Doctoral Dissertations. 1629.