Doctor of Philosophy (PhD)
Polycyclic aromatic amines (arylamines) are a class of chemical carcinogens that are prevalent in environmental and industrial settings. In order to study the mechanism of their toxicity a quantitative and qualitative detection method was developed to measure the C8-adenine adducts of benzidine and 2-aminofluorene in DNA samples. To do this a novel synthetic method using a palladium catalyst was developed to prepare authentic and deuterated arylamine adducts to serve as standards. These standards were then used to develop a high performance liquid chromatography, electrospray, tandem mass spectrometry, isotope dilution, detection method. To demonstrate the validity of this method two spike and recovery experiments were performed.
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Release the entire work immediately for access worldwide.
Olsen, Philip Dmitri, "The synthesis of deuterated arylamine DNA adducts for use in the development of an isotope dilution LC/MS/MS method" (2001). LSU Doctoral Dissertations. 1629.