Microchip electrochemical immunosensor fabricated using micromachining techniques
Using micromachining techniques, an electrochemical immunosensor has been designed, fabricated and tested on a Pyrex glass wafer. The sensor has an electrochemical cell that is composed of a reference, an auxiliary and two working electrodes on a spin-coated polyimide film, where the reference electrode is silver and the others are platinum. The polyimide film is used for antibody immobilization instead of a polystyrene sheet which is usually used in conventional immunosensors. A reaction chamber is fabricated separately on a silicon wafer using anisotropic etching techniques. The electrochemical cell on a glass wafer and the reaction chamber on a silicon wafer are then bonded together to construct the immunosensor using polymer bonding techniques. Fabricated immunosensors are tested by the method of cyclic voltammetry (CV) before analyzing the antigen-antibody reaction. Test antibodies are well attached to the polyimide substrate, and the results of the CV test shows that this immunosensor can be successfully applied for electrochemical immunoassay.
Publication Source (Journal or Book title)
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Choi, J., Ding, Y., Ahn, C., Halsall, H., & Heineman, W. (1997). Microchip electrochemical immunosensor fabricated using micromachining techniques. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, 5, 2264-2266. Retrieved from https://digitalcommons.lsu.edu/eecs_pubs/116