Title

RNA sampling from tissue sections using infrared laser ablation

Document Type

Article

Publication Date

7-31-2019

Abstract

© 2019 Elsevier B.V. RNA was obtained from discrete locations of frozen rat brain tissue sections through infrared (IR) laser ablation using a 3-μm wavelength in transmission geometry. The ablated plume was captured in a microcentrifuge tube containing RNAse-free buffer and processed using a commercial RNA purification kit. RNA transfer efficiency and integrity were evaluated based on automated electrophoresis in microfluidic chips. Reproducible IR-laser ablation of intact RNA was demonstrated with purified RNA at laser fluences of 3–5 kJ/m 2 (72 ± 12% transfer efficiency) and with tissue sections at a laser fluence of 13 kJ/m 2 (79 ± 14% transfer efficiency); laser energies were attenuated ∼20% by the soda-lime glass slides used to support the samples. RNA integrity from tissue ablation was >90% of its original RIN value (∼7) and the purified RNA was sufficiently intact for conversion to cDNA and subsequent qPCR assay.

Publication Source (Journal or Book title)

Analytica Chimica Acta

First Page

91

Last Page

98

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