Title
High-field 19.6 T 27Al solid-state MAS NMR of in vitro aluminated brain tissue
Document Type
Article
Publication Date
10-1-2004
Abstract
The combination of 27Al high-field solid-state NMR (19.6 T) with rapid spinning speeds (17.8 kHz) is used to acquire 27Al NMR spectra of total RNA human brain temporal lobe tissues exposed to 0.10mM Al 3+ (as AlCl3) and of human retinal pigment epithelial cells (ARPE-19), grown in 0.10mM AlCl3. The spectra of these model systems show multiple Al3+ binding sites, good signal/ noise ratios and apparent chemical shift dispersions. A single broad peak (-3 to 11 ppm) is seen for the aluminated ARPE-19 cells, consistent with reported solution-state NMR chemical shifts of Al-transferrin. The aluminated brain tissue has a considerably different 27Al MAS NMR spectrum. In addition to the transferrin-type resonance, additional peaks are seen. Tentative assignments include: -9 to -3 ppm, octahedral AlO6 (phosphate and water); 9 ppm, condensed AlO6 units (Al-O-Al bridges); 24 ppm, tetrahedral AlO 3N and/or octahedral Al-carbonate; and 35 ppm, more N-substituted aluminum and /or tetrahedral Alo4. Thus, brain tissue is susceptible to a broad range of coordination by aluminum. Furthermore, the moderate 27Al CQ values (all less than 10 MHz) suggest future NMR studies may be performed at 9.4 T and a spin rate of 20kHz. © 2004 Elsevier Inc. All rights reserved.
Publication Source (Journal or Book title)
Journal of Magnetic Resonance
First Page
257
Last Page
262
Recommended Citation
Bryant, P., Lukiw, W., Gan, Z., Hall, R., & Butler, L. (2004). High-field 19.6 T 27Al solid-state MAS NMR of in vitro aluminated brain tissue. Journal of Magnetic Resonance, 170 (2), 257-262. https://doi.org/10.1016/j.jmr.2003.12.013