Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity
© The Royal Society of Chemistry. We report a new turn-on substrate probe whose intense fluorescent reporter signature is selectively provided upon probe activation by the cancer-associated oxidoreductase, hNQO1. The extremely high fluorescence turn-on of the probe was utilized to generate fluorescence microscope images of hNQO1-expressing, tumor-derived colorectal and ovarian cancer cells with unprecedented positive signal-to-negative background ratios (PNRs), a key step toward probe application in guided surgical removal of diseased tissues.
Publication Source (Journal or Book title)
Best, Q., Prasai, B., Rouillere, A., Johnson, A., & McCarley, R. (2017). Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity. Chemical Communications, 53 (4), 783-786. https://doi.org/10.1039/C6CC08306D