Title

Probing the reaction coordinate for ligand binding in hemoglobin using ultrafast transient Raman spectroscopy

Document Type

Conference Proceeding

Publication Date

5-1-1993

Abstract

© 1893 SPIE. Transient picosecond Raman spectroscopy is capable of differentiating vibrational relaxation from conformational changes by comparing the Stokes and anti-Stokes dynamics. We report pump-probe picosecond Raman experiments on oxy-and deoxyhemoglobin (oxyHb and deoxyHb, respectively) using 8 ps 532 nm pump pulses and 8 ps 355 nm probe pulses. Heme-to-protein vibrational cooling has been directly observed in deoxyHb for the first time, and the deconvolved cooling time constant is measured to be 2-5 ps.1-2 By applying our mode-specific Stokes and anti-Stokes technique to oxyHb, we find that any geminate recombination of photodeligated O2 must occur in either less than two picoseconds or longer than a nanosecond.

Publication Source (Journal or Book title)

Proceedings of SPIE - The International Society for Optical Engineering

First Page

123

Last Page

126

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