Title
Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM)
Document Type
Article
Publication Date
8-29-2002
Abstract
Chemically selective imaging of human hepatocytes via intrinsic vibrational resonances with spatial resolution below the diffraction limit was carried out. The combination of coherent anti-Stoke Raman scattering (CARS) with near-field scanning optical microscopy (NSOM) was used for the imaging. Femtosecond, near-IR pulses produced CARS signals from hepatocytes and the signal was tuned to be resonant with C-H stretching frequencies. Results showed an image contrast with an optical spatial resolution ∼128 nm.
Publication Source (Journal or Book title)
Journal of Physical Chemistry B
First Page
8489
Last Page
8492
Recommended Citation
Schaller, R., Ziegelbauer, J., Lee, L., Haber, L., & Saykally, R. (2002). Chemically selective imaging of subcellular structure in human hepatocytes with coherent anti-stokes raman scattering (CARS) near-field scanning optical microscopy (NSOM). Journal of Physical Chemistry B, 106 (34), 8489-8492. https://doi.org/10.1021/jp020855t