Title

Preliminary evaluation of mixed dyes for fingerprinting non-fluorescent bacteria

Document Type

Article

Publication Date

1-1-1981

Abstract

A rapid scanning fluorometer is used to obtain fluorescence spectral data characteristic of bacteria which do not readily produce fluorescent pigments. The data is generated in the form of an array of fluorescence intensity as a function of multiple excitation and emission wavelengths. Fluorescence spectral properties are introduced into the bacterial specimen using multiple dye mixtures for staining. The characteristic fluorescent fingerprints are believed to occur by preferential adsorption and reaction of bacterial cellular components with the individual dyes of the mixture. The procedure is found to be rapid and reproducible for the bacteria examined. Copyright © 1981, Taylor & Francis Group, LLC. All rights reserved.

Publication Source (Journal or Book title)

Analytical Letters

First Page

1111

Last Page

1124

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