Novel instrument for measurement of multidimensional fluorescence detected circular dichroism
A new microprocessor-controlled multichannel spectrometer for the measurement of fluorescence detected circular dichroism (FDCD) is presented. Spectra of model steroid compounds are provided to demonstrate the analytical utility of multichannel FDCD over the classical circular dichroism measurement. A detection limit of 3.05×10-8 M was determined for a solution of estrone based on a signal/noise ratio of 2 as a lower limit. The optics and electronics for the rapid acquisition of multichannel FDCD data are described in detail.
Publication Source (Journal or Book title)
Review of Scientific Instruments
Thomas, M., Patonay, G., & Warner, I. (1986). Novel instrument for measurement of multidimensional fluorescence detected circular dichroism. Review of Scientific Instruments, 57 (7), 1308-1313. https://doi.org/10.1063/1.1138594