Purification, partial characterization, and seroreactivity of a genuswide 60-kilodalton Legionella protein antigen
A genuswide protein antigen extracted from Legionella pneumophila serogroup 1 (strain Philadelphia 1) cells was enriched by differential pelleting and ammonium sulfate precipitation and subsequently purified with a combination of high-performance size-exclusion and ion-exchange chromatography. The protein has an apparent molecular weight of 650,000 before and 63,000 after urea (5 M) treatment, as determined by size-exclusion chromatography. These proteins resolved to a single band of 60,000 after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The urea-treated protein had an isoelectric point of 5.8. This purified 60-kilodalton protein reacted with a convalescent-phase serum sample from a patient with legionellosis and rabbit immune sera prepared against each of 23 Legionella species. The 60-kilodalton protein may be useful in developing diagnostic tests for legionellosis.
Publication Source (Journal or Book title)
Journal of Clinical Microbiology
Chou-Pong Pau., Plikaytis, B., Carlone, G., & Warner, I. (1988). Purification, partial characterization, and seroreactivity of a genuswide 60-kilodalton Legionella protein antigen. Journal of Clinical Microbiology, 26 (1), 67-71. https://doi.org/10.1128/jcm.26.1.67-71.1988