Determination of Cyclodextrin Formation Constants Using Dynamic Coupled-Column Liquid Chromatography
Dynamic coupled-column liquid chromatography is used to determine both aqueous solubilities and cyclodextrin inclusion complex formation constants for a series of polynuclear aromatic hydrocarbons (PAHs). This method Is based on the increase In solubility afforded by cyclodextrlns upon complexation. Formation constant (Kf) values are determined by using α-, β-, and γ-cyclodextrlns at various temperatures. The relative precision of the calculated Kf values is best for cyclodextrin/PAH complexes with large formation constants. The calculated solubilities are comparable to literature values, and in most cases, formation constant data are reproducible within 10%. The resulting Kf values confirm that the formation of Inclusion complexes is based on the relative sizes of host and guest molecules. © 1989, American Chemical Society. All rights reserved.
Publication Source (Journal or Book title)
Blyshak, L., Dodson, K., Patonay, G., Warner, I., & May, W. (1989). Determination of Cyclodextrin Formation Constants Using Dynamic Coupled-Column Liquid Chromatography. Analytical Chemistry, 61 (9), 955-960. https://doi.org/10.1021/ac00184a008