Retention behavior of β-cyclodextrin complexes of anthracene and pyrene using reversed-phase liquid chromatography. The effects of tert.-butanol and cyclopentanol
The effects of tert.-butanol and cyclopentanol on the formation of β-cyclodextrin (β-CD)-anthracene and β-CD-pyrene complexes have been studied using reversed-phase liquid chromatography. The retention times of anthracene and pyrene were monitored when eluted in a C18 column with mobile phase mixtures consisting of methanol, water, β-CD, and a small amount of tert.-butanol or cyclopentanol. Plots of capacity factor of anthracene versus concentration of β-CD in the mobile phase in methanol-water mixtures and in mixtures containing 1% (v/v) cyclopentanol or tert.-butanol as secondary modifiers showed very similar trends. This suggests that anthracene forms weak complexes with β-CD, and that the presence of these secondary mobile phase modifiers have little effect on the β-CD-anthracene inclusion reaction. In contrast, pyrene showed no discernable change in retention time with increases in β-CD concentration. However, in the presence of 1% (v/v) tert.-butanol or cyclopentanol, capacity factor of pyrene decreased drastically, more in 1% (v/v) cyclopentanol than in 1% (v/v) tert.-butanol. Formation of a ternary β-CD-pyrene-alcohol complex has been proposed to explain the effects. © 1992.
Publication Source (Journal or Book title)
Journal of Chromatography A
Anigbogu, V., de la Peña, A., Ndou, T., & Warner, I. (1992). Retention behavior of β-cyclodextrin complexes of anthracene and pyrene using reversed-phase liquid chromatography. The effects of tert.-butanol and cyclopentanol. Journal of Chromatography A, 594 (1-2), 37-43. https://doi.org/10.1016/0021-9673(92)80311-H