Separation of water-soluble p-sulfonated calixarenes 4, 6 and 8 and 4-hydroxybenzene sulfonate by use of capillary zone electrophoresis
A method for the separation of sulfonated calixarenes 4, 6 and 8 and the 4-hydroxybenzene sulfonate monomer by capillary zone electrophoresis with direct UV absorbance detection is described. The electroosmotic flow (EOF) decreases with addition of K+ and reverses with addition of Mg2+. The suppression of the EOF upon the addition of K+ slightly improves the separation of the calixarenes and the monomer. However, the elution times of those compounds increase almost 35 min. The reversal of the EOF by addition of Mg2+ reduces the migration time and also improves the separation efficiency. The calixarenes are baseline separated in less than 14 min by use of a borate buffer with the addition of 4.0. 6.0 or 12.0 mM MgCl2, at pH 8.3. © 1994.
Publication Source (Journal or Book title)
Journal of Chromatography A
Zhang, Y., & Warner, I. (1994). Separation of water-soluble p-sulfonated calixarenes 4, 6 and 8 and 4-hydroxybenzene sulfonate by use of capillary zone electrophoresis. Journal of Chromatography A, 688 (1-2), 293-300. https://doi.org/10.1016/0021-9673(94)00816-7