Effect of co-modifiers in cyclodextrin-modified mobile phases on the reversed-phase high-performance liquid chromatographic separation of polyaromatic hydrocarbons

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The effect of two co-modifier containing tert.-butyl moieties, tert.-butyl (N-hydroxy) carbamate and tert.-butyl carbazate, on the retention behavior of six polyaromatic hydrocarbons (PAHs) using cyclodextrin-modified mobile phases has been examined. Both modifiers resulted in shorter retention times for all PAHs, when a size-compatible cyclodextrin was present in the eluent. This decrease has been attributed to the formation of a ternary PAH-cyclodextrin-modifier complex. The apparent formation constants calculated for these complexes are of the order of approximately 102 M-1, with the exception of the β-CD-pyrene complex, which was determined to have a 2:1 stoichiometry. Mixtures of β- and γ-cyclodextrins were used in the mobile phase along with tert.-butyl carbazate to achieve a baseline separation of all six PAHs under 50 min. © 1995.

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Journal of Chromatography A

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