Effect of co-modifiers in cyclodextrin-modified mobile phases on the reversed-phase high-performance liquid chromatographic separation of polyaromatic hydrocarbons
The effect of two co-modifier containing tert.-butyl moieties, tert.-butyl (N-hydroxy) carbamate and tert.-butyl carbazate, on the retention behavior of six polyaromatic hydrocarbons (PAHs) using cyclodextrin-modified mobile phases has been examined. Both modifiers resulted in shorter retention times for all PAHs, when a size-compatible cyclodextrin was present in the eluent. This decrease has been attributed to the formation of a ternary PAH-cyclodextrin-modifier complex. The apparent formation constants calculated for these complexes are of the order of approximately 102 M-1, with the exception of the β-CD-pyrene complex, which was determined to have a 2:1 stoichiometry. Mixtures of β- and γ-cyclodextrins were used in the mobile phase along with tert.-butyl carbazate to achieve a baseline separation of all six PAHs under 50 min. © 1995.
Publication Source (Journal or Book title)
Journal of Chromatography A
Husain, N., Christian, A., & Warner, I. (1995). Effect of co-modifiers in cyclodextrin-modified mobile phases on the reversed-phase high-performance liquid chromatographic separation of polyaromatic hydrocarbons. Journal of Chromatography A, 699 (1-2), 73-83. https://doi.org/10.1016/0021-9673(95)00038-O