On-line capillary electrophoresis-electrospray ionization mass spectrometry using a polymerized anionic surfactant
On-line capillary electrophoresis - electrospray ionization - mass spectrometry (CE-ESI-MS) has been used to determine the tricyclic antidepressant drugs (imipramine, doxepin, and amitriptyline) as well as the β-adrenergic blocker drugs (propranolol and alprenolol). A CE-ESI-MS interface linking a manually operated CE system and a Finnigan MAT-900 sector mass spectrometer (with an Analytica electrospray ionization source) has been constructed in-house and employed for this study. Although a water/methanol based capillary zone electrophoresis (CZE) buffer was initially used to determine these analytes, enhanced resolution was obtained by addition of a polymerized surfactant, i.e., poly-sodium undecylenic sulfate (poly-SUS), into the electrokinetic chromatography (EKC) buffer. When a low concentration of this poly-SUS surfactant was added to a volatile EKC buffer, these structurally similar cationic drugs were EKC separated and on-line detected by ESI-MS.
Publication Source (Journal or Book title)
Lu, W., Shamsi, S., McCarley, T., & Warner, I. (1998). On-line capillary electrophoresis-electrospray ionization mass spectrometry using a polymerized anionic surfactant. Electrophoresis, 19 (12), 2193-2199. https://doi.org/10.1002/elps.1150191225