Separation and identification of chiral N-acylcalixarene amino acid derivatives by use of reversed-phase HPLC
This work focuses on the separation and identification of p-tert butyl calixarene derivatives. An isocratic mixture of 65 %(v/v) acetonitrile and 35 %(v/v) water with 0.1 % (v/v) phosphoric acid was used as the mobile phase. A Bio-Rad Bio-Sil ODS-5S (250 mm x 4 mm) column was used as the stationary phase with UV detection of the analytes at 274 nm. The reversed- phase high performance liquid chromatographic method which was developed provided baseline separation of five p-tert-butyl calixarene derivatives in twenty minutes.
Publication Source (Journal or Book title)
Thibodeaux, S., Sañchez Peña, M., Zhang, Y., Shamsi, S., & Warner, I. (1999). Separation and identification of chiral N-acylcalixarene amino acid derivatives by use of reversed-phase HPLC. Chromatographia, 49 (3-4), 142-146. https://doi.org/10.1007/BF02575276