Membrane on a chip: A functional tethered lipid bilayer membrane on silicon oxide surfaces
Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin. © 2005 by the Biophysical Society.
Publication Source (Journal or Book title)
Atanasov, V., Knorr, N., Duran, R., Ingebrandt, S., Offenhäusser, A., Knoll, W., & Köper, I. (2005). Membrane on a chip: A functional tethered lipid bilayer membrane on silicon oxide surfaces. Biophysical Journal, 89 (3), 1780-1788. https://doi.org/10.1529/biophysj.105.061374