Studies on the subcellular localization of the porphycene CPO
This study was designed to provide more detailed information on the subcellular sites of binding of the porphycene, termed 9-capronyloxytetrakis (methoxyethyl) porphycene (CPO), with a fluorescence resonance energy transfer (FRET) technique. The proximity of CPO to two fluorescent probes was determined: nonyl acridine orange (NAO), a dye with specific affinity for the mitochondrial lipid cardiolipin, and dihexaoxacarbocyanine iodide (DiOC 6), an agent that labels the endoplasmic reticulum (ER). FRET spectra indicated energy transfer between DiOC 6 and CPO but no significant transfer between NAO and CPO. These results confirm data obtained by fluorescence microscopy, suggesting a similar pattern of subcellular localization by CPO and DiOC 6 but not by CPO and NAO. However, when cells containing CPO were irradiated and then loaded with NAO, FRET between the two fluorophores was observed. Hence, a relocalization of CPO can occur during irradiation. These data provide an explanation for recent studies on CPO-catalyzed photodamage to both ER and mitochondrial Bcl-2. © 2005 American Society for Photobiology.
Publication Source (Journal or Book title)
Photochemistry and Photobiology
Kessel, D., Conley, M., Vicente, M., & Reiners, J. (2005). Studies on the subcellular localization of the porphycene CPO. Photochemistry and Photobiology, 81 (3), 569-572. https://doi.org/10.1562/2004-12-16-RA-403.1