Synthesis and biological investigations of a ZnPc-antiCEA bioconjugate for imaging of colorectal cancer
Two zinc(II) phthalocyanines (ZnPcs) were conjugated with a monoclonal antibody (MAb) directed against carcinoembryonic antigen (CEA), using an in situ activated carboxylic acid on the ZnPcs. The bioconjugate with the highest ZnPc/MAb ratio of 3 was investigated in vitro for its ability to target and fluorescently label human colorectal HT-29 cells. The ZnPc-CEA MAb 2 was observed to efficiently target HT-29 cells, about 37 times more than unconjugated ZnPc. Furthermore, in the presence of a 4-fold excess of unlabelled anti-CEA antibody, the fluorescence signal of 2 was reduced by ∼90% showing that the targeting is CEA-mediated. These studies further confirm the high specificity of Pc-antibody conjugates for antigens over-expressed on tumor cells and warrant further investigations of these immunoconjugates and their derivatives for imaging of colorectal cancer. Copyright © 2013 World Scientific Publishing Company.
Publication Source (Journal or Book title)
Journal of Porphyrins and Phthalocyanines
Sehgal, I., Li, H., Ongarora, B., Devillier, D., & Vicente, M. (2013). Synthesis and biological investigations of a ZnPc-antiCEA bioconjugate for imaging of colorectal cancer. Journal of Porphyrins and Phthalocyanines, 17 (1-2), 150-156. https://doi.org/10.1142/S108842461250143X