Title

Synthesis and biological investigations of a ZnPc-antiCEA bioconjugate for imaging of colorectal cancer

Document Type

Article

Publication Date

1-1-2013

Abstract

Two zinc(II) phthalocyanines (ZnPcs) were conjugated with a monoclonal antibody (MAb) directed against carcinoembryonic antigen (CEA), using an in situ activated carboxylic acid on the ZnPcs. The bioconjugate with the highest ZnPc/MAb ratio of 3 was investigated in vitro for its ability to target and fluorescently label human colorectal HT-29 cells. The ZnPc-CEA MAb 2 was observed to efficiently target HT-29 cells, about 37 times more than unconjugated ZnPc. Furthermore, in the presence of a 4-fold excess of unlabelled anti-CEA antibody, the fluorescence signal of 2 was reduced by ∼90% showing that the targeting is CEA-mediated. These studies further confirm the high specificity of Pc-antibody conjugates for antigens over-expressed on tumor cells and warrant further investigations of these immunoconjugates and their derivatives for imaging of colorectal cancer. Copyright © 2013 World Scientific Publishing Company.

Publication Source (Journal or Book title)

Journal of Porphyrins and Phthalocyanines

First Page

150

Last Page

156

This document is currently not available here.

Share

COinS