Title

Spatial distribution, localization, and speciation of arsenic in the hyperaccumulating fern Pteris vittata L.

Document Type

Article

Publication Date

12-1-2007

Abstract

This study is concerned with the distribution, localization, and speciation of arsenic (As) in the hyperaccumulating fern Pteris vittata L. Sections across an intact pinnae, stipe, and root were taken from plants grown in soil treated with 1% or 200 ppm As. Arsenic distribution and speciation in plant material were examined with micro-focused X-rays and X-ray absorption near-edge structure (XANES). In the pinnae, As was confined to the midrib and minor veins, especially those in close proximity to the blade margins. Very little As was detected in the cellular compartments of fresh root or stipe sections, whereas in cryosections of the pinnae, As was localized in epidermal cells, mesophyll cells, and xylem tissues. XANES showed that As occurred predominantly as arsenite [As(III), the most toxic form] in all tissues. Analysis of xylem sap showed that As was present as arsenate (As(V)) and reduced to arsenite (As(III)) in the fronds. P. vittata may adapt to As-rich soils by translocating As as As(V) and then compartmentalizing it as As(III) in the shoot system. Such information could be very valuable for the successful implementation of phytoremediation strategy as well as proper disposal of toxic waste generated after phytoremediating As-contaminated soils. © 2007 Elsevier B.V. All rights reserved.

Publication Source (Journal or Book title)

Trace Metals and other Contaminants in the Environment

First Page

299

Last Page

313

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