Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer
A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions. © 2009 The Royal Society of Chemistry.
Publication Source (Journal or Book title)
Yoshimatsu, K., Lejeune, J., Spivak, D., & Ye, L. (2009). Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer. Analyst, 134 (4), 719-724. https://doi.org/10.1039/b814967d