Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes
S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays as the outermost cell membrane component. These proteins have the unique property that following disruption by chemical agents, monomers of the protein can re-assemble to their original lattice structure. This unique property makes S-layers interesting for utilization in bio-nanotechnological applications. Here, we show that the addition of S-layer proteins to bilayer lipid membranes increases the lifetime and the stability of the bilayer. M2δ ion channels were functionally incorporated into these S-layer stabilized membranes and we were able to record their activity for up to 20 h. Transmission electron microscopy (TEM) was used to visualize the 2D crystalline pattern of the S-layer and the M2δ ion channel characteristics in bilayer lipid membrane's were compared in the presence and absence of S-layers. © 2008 Elsevier B.V.
Publication Source (Journal or Book title)
Colloids and Surfaces B: Biointerfaces
Keizer, H., Andersson, M., Chase, C., Laratta, W., Proemsey, J., Tabb, J., Long, J., & Duran, R. (2008). Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes. Colloids and Surfaces B: Biointerfaces, 65 (2), 178-185. https://doi.org/10.1016/j.colsurfb.2008.04.015