Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes

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S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays as the outermost cell membrane component. These proteins have the unique property that following disruption by chemical agents, monomers of the protein can re-assemble to their original lattice structure. This unique property makes S-layers interesting for utilization in bio-nanotechnological applications. Here, we show that the addition of S-layer proteins to bilayer lipid membranes increases the lifetime and the stability of the bilayer. M2δ ion channels were functionally incorporated into these S-layer stabilized membranes and we were able to record their activity for up to 20 h. Transmission electron microscopy (TEM) was used to visualize the 2D crystalline pattern of the S-layer and the M2δ ion channel characteristics in bilayer lipid membrane's were compared in the presence and absence of S-layers. © 2008 Elsevier B.V.

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Colloids and Surfaces B: Biointerfaces

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