Document Type
Article
Publication Date
8-1-2010
Abstract
Five cationic porphyrins bearing one to four -N(CH3)3+ groups linked to the p-phenyl positions of 5,10,15,20-tetraphenylporphyrin (TPP) were synthesized in order to study the effect of overall charge and its distribution on the cellular uptake, phototoxicity and intracellular localization using human carcinoma HEp2 cells. The di-cationic porphyrins DADP-o and DADP-a accumulated the most within cells and preferentially localize within vesicular compartments and in mitochondria. Of these two only DADP-a was phototoxic to the cells (IC50=3μM at 1J/cm2). The mono-cationic porphyrin MAP was found to be the most phototoxic of the series, and it localized mainly in lipid membranes, including the plasma membrane, ER, mitochondria, and Golgi. Both the tri-cationic porphyrin TRAP and the tetra-cationic porphyrin TEAP localized subcellularly mainly in the mitochondria, but of the two only TEAP showed moderate phototoxicity (IC50=8μM at 1J/cm2). Our results suggest that MAP is the most promising PDT photosensitizer, and that both DADP-o and TRAP might find application as transport vehicles for therapeutics into cells. © 2010 Elsevier B.V.
Publication Source (Journal or Book title)
Journal of Photochemistry and Photobiology B: Biology
First Page
100
Last Page
111
Recommended Citation
Jensen, T., Vicente, M., Luguya, R., Norton, J., Fronczek, F., & Smith, K. (2010). Effect of overall charge and charge distribution on cellular uptake, distribution and phototoxicity of cationic porphyrins in HEp2 cells. Journal of Photochemistry and Photobiology B: Biology, 100 (2), 100-111. https://doi.org/10.1016/j.jphotobiol.2010.05.007