Hydration and Localization of Diacylglycerol in the Insect Lipoprotein Lipophorin. A 13C-NMR Study
In order to probe the organization of diacylglycerol (DG) in lipophorin, 13C-enriched lipophorin was prepared for NMR investigations. We obtained 13C-enriched lipophorin labeled exclusively in DG by feeding insects tobacco leaves coated with [1-13C]palmitic acid or [l-13C]oleic acid. Lipophorins enriched up to 5% with a [13C] fatty acid were obtained by this procedure. NMR studies of the isolated lipophorin DG showed that palmitic acid accumulates almost entirely (>90%) in the sn-1 position. Oleic acid was found equally distributed between the sn-1 and sn-2 positions, yielding a DG enriched equally at both positions. The 13C-NMR spectra of both [13C]palmitate- and [13C]oleate-enriched lipophorins showed that DG had one narrow carbonyl resonance indicative of rapid motion. A comparative analysis of the 13C carbonyl chemical shift data for DG in organic solvents, aqueous solutions, and dispersions with the DG carbonyl chemical shift of native lipophorin enriched in [13C]palmitate or [13C]oleate shows a high degree of water exclusion from the DG carbonyls in lipophorin. This result is consistent with the existence of a lipophorin lipid core containing most of the lipophorin DG. This study represents the First attempt to elucidate the organization of DG in lipophorin. The possibility of obtaining [13C]DG-enriched lipophorins, selectively enriched in one or both acyl chains of DG, should provide a powerful tool for further analysis of the organization and the dynamic properties of DG in native lipoproteins. © 1994, American Chemical Society. All rights reserved.
Publication Source (Journal or Book title)
Soulages, J., Wells, M., Rivera, M., & Walker, F. (1994). Hydration and Localization of Diacylglycerol in the Insect Lipoprotein Lipophorin. A 13C-NMR Study. Biochemistry, 33 (11), 3245-3251. https://doi.org/10.1021/bi00177a015