In situ polymerization of PEGDA foam for bone defects
© Copyright 2015 by ASME. The purpose of this study is to develop a novel bone replacement using in situ polymerization of thiol-acrylate with adipose tissue derived adult stem cells (ASCs). Specifically, Poly(ethylene glycol) diacrylate-co-trimethylolpropane tris (3-mercaptopropionate) (PEGDA-co-TMPTMP) was synthesized with 10%Hydroxyapatite (HA) foam by an amine-catalyzed Michael addition reaction. Initial characterization studies were performed to determine the temperature profile during the exothermic reaction showing a peak temperature of 50°C. To prevent hyperthermic cell damage and death during the exothermic polymerization procedure, the hASCs were encapsulated in alginate. Characterization of the 3-D structure and interconnectivity of pores in the polymeric foam scaffolds were performed using FIB-SEM and Micro-CT showing uniform distribution of HA. Cell viability experiments within the polymeric scaffold were performed using Vybrant® MTT cell profileration method, as well as fluorescent dyes: Calcein-AM (live) and Ethidium homodimer-1 (dead) showing viability of cells inside the samples.
Publication Source (Journal or Book title)
ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Forghani, A., Garber, L., Chen, C., Devireddy, R., Pojman, J., & Hayes, D. (2015). In situ polymerization of PEGDA foam for bone defects. ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 3-2015 https://doi.org/10.1115/IMECE2015-51235