Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads
© 2019 Author(s). In theory, groups of enzyme-loaded particles producing an acid or base may show complex behavior including dynamical quorum sensing, the appearance of synchronized oscillations above a critical number or density of particles. Here, experiments were performed with the enzyme urease loaded into mm-sized agarose beads and placed in a solution of urea, resulting in an increase in pH. This behavior was found to be dependent upon the number of beads present in the array; however, reaction-induced convection occurred and plumes of high pH developed that extended to the walls of the reactor. The convection resulted in the motion of the mm-sized particles and conversion of the solution to high pH. Simulations in a simple model of the beads demonstrated the suppression of dynamical quorum sensing in the presence of flow.
Publication Source (Journal or Book title)
Markovic, V., Bánsági, T., McKenzie, D., Mai, A., Pojman, J., & Taylor, A. (2019). Influence of reaction-induced convection on quorum sensing in enzyme-loaded agarose beads. Chaos, 29 (3) https://doi.org/10.1063/1.5089295