Development of a flow-free gradient generator using a self-adhesive thiol-acrylate microfluidic resin/hydrogel (TAMR/H) hybrid system
© 2020 CBMS-0001 Microfluidic gradient generators can be used to study cellular migration, growth, and drug response in numerous biological systems. Several different types of flow-free devices have been fabricated consisting of hydrogels coupled with polydimethylsiloxane (PDMS); however, the operation of these PDMS/hydrogel devices is difficult due to the lack of adhesive interactions requiring external clamping. This work utilizes novel polymeric materials using thiol-ene chemistry to fabricate a two-layer, flow-free microfluidic gradient to couple the top fluidic-layer with the bottom hydrogel-layer allowing for an orthogonal chemical gradient. As proof of concept, the device was used to study the chemotactic response of E. coli.
Publication Source (Journal or Book title)
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Khan, A., Smith, N., Tullier, M., Seth Roberts, B., Englert, D., Pojman, J., & Melvin, A. (2020). Development of a flow-free gradient generator using a self-adhesive thiol-acrylate microfluidic resin/hydrogel (TAMR/H) hybrid system. MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 406-407. Retrieved from https://digitalcommons.lsu.edu/chemistry_pubs/1085