Development of a flow-free gradient generator using a self-adhesive thiol-acrylate microfluidic resin/hydrogel (TAMR/H) hybrid system

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Conference Proceeding

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© 2020 CBMS-0001 Microfluidic gradient generators can be used to study cellular migration, growth, and drug response in numerous biological systems. Several different types of flow-free devices have been fabricated consisting of hydrogels coupled with polydimethylsiloxane (PDMS); however, the operation of these PDMS/hydrogel devices is difficult due to the lack of adhesive interactions requiring external clamping. This work utilizes novel polymeric materials using thiol-ene chemistry to fabricate a two-layer, flow-free microfluidic gradient to couple the top fluidic-layer with the bottom hydrogel-layer allowing for an orthogonal chemical gradient. As proof of concept, the device was used to study the chemotactic response of E. coli.

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MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

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