Title

Reproducibility and quantitation of matrix‐assisted laser desorption ionization mass spectrometry: Effects of nitrocellulose on peptide ion yields

Document Type

Article

Publication Date

1-1-1993

Abstract

We have observed that using nitrocellulose in sample preparation for matrix‐assisted laser desorption ionization (MALDI) time‐of‐flight mass spectrometry increases the yield of peptide [M + H]+ ions. Addition of nitrocellulose also provides improvement in sample‐to‐sample reproducibility of MALDI ion yield and improves the precision of peptide quantitation. Mass spectrometry and optical microscopy results suggest that the nitrocellulose modifies the crystallization of matrix/analyte solution to allow more even coverage over the sample surface. Copyright © 1993 John Wiley & Sons, Ltd.

Publication Source (Journal or Book title)

Biological Mass Spectrometry

First Page

544

Last Page

550

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