On-Line Coupling of Gel Permeation Chromatography with MALDI Mass Spectrometry
Gel permeation chromatography (GPC) was combined directly with aerosol matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry for the analysis of synthetic polymers. In the aerosol MALDI/GPC-MS experiment, effluent from the GPC column was combined with matrix solution and sprayed directly into a time-of-flight mass spectrometer (TOF-MS). Ions were formed by irradiation of the aerosol particles with pulsed 355 nm radiation from a frequency-tripled Nd:YAG laser. The ions were mass separated in a two-stage reflectron TOF-MS, and averaged mass spectra were stored every 11s throughout the GPC-MS experiment On-line GPC/ MALDI-MS was performed with the commercially available polymers poly(ethylene glycol) (PEG 1000) and poly(propylene glycol) (PPG 1000).
Publication Source (Journal or Book title)
Fei, X., & Murray, K. (1996). On-Line Coupling of Gel Permeation Chromatography with MALDI Mass Spectrometry. Analytical Chemistry, 68 (20), 3555-3560. https://doi.org/10.1021/ac960352h