Title
On-Line Coupling of Gel Permeation Chromatography with MALDI Mass Spectrometry
Document Type
Article
Publication Date
10-15-1996
Abstract
Gel permeation chromatography (GPC) was combined directly with aerosol matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry for the analysis of synthetic polymers. In the aerosol MALDI/GPC-MS experiment, effluent from the GPC column was combined with matrix solution and sprayed directly into a time-of-flight mass spectrometer (TOF-MS). Ions were formed by irradiation of the aerosol particles with pulsed 355 nm radiation from a frequency-tripled Nd:YAG laser. The ions were mass separated in a two-stage reflectron TOF-MS, and averaged mass spectra were stored every 11s throughout the GPC-MS experiment On-line GPC/ MALDI-MS was performed with the commercially available polymers poly(ethylene glycol) (PEG 1000) and poly(propylene glycol) (PPG 1000).
Publication Source (Journal or Book title)
Analytical Chemistry
First Page
3555
Last Page
3560
Recommended Citation
Fei, X., & Murray, K. (1996). On-Line Coupling of Gel Permeation Chromatography with MALDI Mass Spectrometry. Analytical Chemistry, 68 (20), 3555-3560. https://doi.org/10.1021/ac960352h