Aerosol MALDI with a reflectron time-of-flight mass spectrometer
Aerosol matrix-assisted laser desorption/ionization (MALDI) has been combined with a reflectron time-of-flight mass spectrometer for improved mass resolution. A methanol solution of matrix and analyte was sprayed directly into a reflectron time-of-flight mass spectrometer, and the aerosol particles were irradiated and ionized with a frequency-tripled Nd:YAG laser at 355 nm. Mass resolution of over 300 was observed for the peptides bradykinin, angiotensin II, and gramicidin D and for vitamin B12. This represents a resolution enhancement of approximately 10-fold over that previously reported for aerosol MALDI with a linear time-of-flight instrument.
Publication Source (Journal or Book title)
Fei, X., Wei, G., & Murray, K. (1996). Aerosol MALDI with a reflectron time-of-flight mass spectrometer. Analytical Chemistry, 68 (7), 1143-1147. Retrieved from https://digitalcommons.lsu.edu/chemistry_pubs/1055