Title

Two-laser infrared and ultraviolet matrix-assisted laser desorption/ionization

Document Type

Article

Publication Date

7-1-2003

Abstract

Matrix-assisted laser desorption/ionization (MALDI) was performed using two pulsed lasers with wavelengths in the IR and UV regions. A 10.6 μm pulsed CO2 laser was used to irradiate a MALDI target, followed after an adjustable delay by a 337 nm pulsed nitrogen laser. The sample consisted of a 2,5-dihydroxybenzoic acid matrix and bovine insulin guest molecule. The pulse energy for both of the lasers was adjusted so that the ion of interest, either the matrix or guest ion, was not produced by either of the lasers alone. The delay time for maximum ion yield occurs at 1 μs for matrix and guest ions and the signal decayed to zero in ∼400 μs. A mechanism is presented for enhanced UV MALDI ion yield following the IR laser pulse based on transient heating. Copyright © 2003 John Wiley & Sons, Ltd.

Publication Source (Journal or Book title)

Journal of Mass Spectrometry

First Page

772

Last Page

777

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