Differential display: Identifying genes involved in cardiomyocyte proliferation
An estimated 15,000 different mRNA species are expressed in a typical mammalian cell. The differential expression of mRNAs in both a temporal and cell-specific manner determines the fate of the cell and creates the organism. Analysis of this differential gene expression has become a central aim of many laboratories attempting to understand the mechanisms underlying various biological processes. Currently, we are using a technique called differential display to analyze the differential expression of genes in cardiomyocytes. Differential display is a rapid and powerful technique that was introduced by Liang and Pardee in 1992. Since that time, it has been successfully applied by several groups, and it is quickly becoming a standard method for studying differential gene expression. Here, we present a detailed article discussing the differential display methodology and how we have utilized it to identify potential genes involved in cardiomyocyte proliferation. Furthermore, we have provided a list of materials and supplied examples of data obtained, in an effort to allow the reader to perform the technique with success in their own laboratory.
Publication Source (Journal or Book title)
Molecular and Cellular Biochemistry
Cormier-Regard, S., Egeland, D., Tannoch, V., & Claycomb, W. (1997). Differential display: Identifying genes involved in cardiomyocyte proliferation. Molecular and Cellular Biochemistry, 172 (1-2), 111-120. https://doi.org/10.1023/A:1006819705814