The relationship between eosinophils and the development of Ag-induced pulmonary pathologies, including airway hyper-responsiveness, was investigated using mice deficient for the secondary granule component, major basic protein-1 (mMBP-1). The loss of mMBP-1 had no effect on OVA-induced airway histopathologies or inflammatory cell recruitment. Lung function measurements of knockout mice demonstrated a generalized hyporeactivity to methacholine-induced airflow changes (relative to wild type); however, this baseline phenotype was observable only with methacholine; no relative airflow changes were observed in response to another nonspecific stimulus (serotonin). Moreover, OVA sensitization/aerosol challenge of wild-type and mMBP-1(-/-) mice resulted in identical dose-response changes to either methacholine or serotonin. Thus, the airway hyper-responsiveness in murine models of asthma occurs in the absence of mMBP-1.
Publication Source (Journal or Book title)
Journal of Immunology
Denzler, K., Farmer, S., Crosby, J., Borchers, M., Cieslewicz, G., Larson, K., Cormier-Regard, S., Lee, N., & Lee, J. (2000). Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma. Journal of Immunology, 165 (10), 5509-5517. https://doi.org/10.4049/jimmunol.165.10.5509