Prenylated Coumaric Acids from Beneficially Modulate Adipogenesis
Two new diprenylated coumaric acid isomers ( and ) and two known congeners, capillartemisin A () and B (), were isolated from as bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant's 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed with extract.
Publication Source (Journal or Book title)
Journal of natural products
Ribnicky, D., Beom Kim, S., Poulev, A., Wang, Y., Boudreau, A., Raskin, I., Bisson, J., & Ray, G. J. (2021). Prenylated Coumaric Acids from Beneficially Modulate Adipogenesis. Journal of natural products, 84 (4), 1078-1086. https://doi.org/10.1021/acs.jnatprod.0c01149