"Myokine Expression in Muscle and Myotubes in Response to Exercise Stim" by Jeffrey D. Covington, Charmaine S. Tam et al.

Faculty Publications

Title

Myokine Expression in Muscle and Myotubes in Response to Exercise Stimulation

Authors

Jeffrey D. Covington, Pennington Biomedical Research Center, Baton Rouge, LA; School of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA; The Charles Perkins Centre and The School of Biological Sciences, University of Sydney, NSW, AUSTRALIA; Department of Kinesiology, University of Texas in El Paso, El Paso, TX; and School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, CHILE.
Charmaine S. Tam
Sudip Bajpeyi
Jose E. Galgani
Robert C. Noland
Steven R. Smith
Leanne M. Redman
Eric Ravussin

Document Type

Article

Publication Date

3-1-2016

Abstract

PURPOSE: Myokines have been shown to affect muscle physiology and exert systemic effects. We endeavored to investigate a panel of myokine mRNA expression after a single exercise bout (studies 1 and 2) to measure myokine mRNA in primary human myotubes in an in vitro exercise model (study 2). METHODS: Vastus lateralis muscle biopsies were obtained from 20 healthy males (age, 24.0 ± 4.5 yr; BMI, 23.6 ± 1.8 kg·m)(-2) before and after a single exercise bout (650 kcal at 50% V˙O2max). Primary myotubes from active and sedentary male donors were treated with a pharmacological cocktail (palmitate, forskolin, and ionomycin (PFI)) to mimic exercise-stimulated contractions in vitro. RESULTS: Interleukin 6 and 8 (IL-6 and IL-8), leukocyte-inducing factor, and connective tissue growth factor (CTGF) mRNA levels increased approximately 10-fold after a single exercise bout (all P < 0.001), whereas myostatin levels decreased (P < 0.05). Key correlations between myokine expression and parameters of muscle and whole-body physiology were found: myostatin versus skeletal muscle citrate synthase activity (r = -0.69, P < 0.001), V˙O2max (r = -0.64, P = 0.002) and the percentage of Type I fibers (r = -0.55, P = 0.01); IL-6 versus the RER (r = 0.45, P = 0.04), homeostatic model assessment of insulin resistance (r = 0.44, P = 0.05), and serum lactate (r = 0.50, P = 0.02). Myokine expressions in myotubes from sedentary donors for CTGF and myostatin decreased, whereas IL-6 and IL-8 increased after PFI treatment. In myotubes from active donors, myokine expression increased for IL-6, CTGF, and myostatin but decreased for IL-8 after PFI treatment. CONCLUSION: These data offer insight into the differences in regulation of myokine expression and their possible physiologic relationships.

Publication Source (Journal or Book title)

Medicine and science in sports and exercise

First Page

384

Last Page

Recommended Citation

Covington, J. D., Tam, C. S., Bajpeyi, S., Galgani, J. E., Noland, R. C., Smith, S. R., Redman, L. M., & Ravussin, E. (2016). Myokine Expression in Muscle and Myotubes in Response to Exercise Stimulation. Medicine and science in sports and exercise, 48 (3), 384-90. https://doi.org/10.1249/MSS.0000000000000787

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