Title

Use of a monoclonal antibody in structural investigations of the 49-kDa polypeptide of photosystem II

Document Type

Article

Publication Date

1-1-1987

Abstract

A monoclonal antibody, FAC2, was isolated by immunization of mice with a Photosystem II core preparation followed by spleenic fusion and standard monoclonal antibody screening and production techniques. This antibody recognizes the 49-kDa polypeptide of Photosystem II which is the apoprotein of CPal. The antigenic determinant recognized by this antibody lies on a cyanogen bromide fragment which appears as a doublet with an apparent molecular mass of 14.5 kDa. FAC2 was used to follow the effects of trypsin on the 49-kDa polypeptide in a membrane environment. Our results indicate that the extrinsic polypeptides of Photosystem II which are known to be involved in oxygen evolution protect the 49-kDa polypeptide from tryptic attack. Additionally, Photosystem II membranes which are treated with alkaline Tris exhibit a large increase in the ability to bind FAC2. This increase is not observed with membranes treated with calcium chloride or sodium chloride. These results indicate that the 49-kDa polypeptide may be at least structurally associated with the component(s) responsible for oxygen evolution. © 1987 Academic Press, Inc. All rights of reproduction in any form reserved.

Publication Source (Journal or Book title)

Archives of Biochemistry and Biophysics

First Page

295

Last Page

301

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