Owl Monkey Insertion Polymorphisms and Phylogenetics
Owl monkeys (genus ), or "night monkeys" are platyrrhine primates in the Aotidae family. Early taxonomy only recognized one species, , until 1983, when Hershkovitz proposed nine unique species designations, classified into red-necked and gray-necked species groups based predominately on pelage coloration. Recent studies questioned this conventional separation of the genus and proposed designations based on the geographical location of wild populations. retrotransposons are a class of mobile element insertion (MEI) widely used to study primate phylogenetics. A scaffold-level genome assembly for one species, [Anan_2.0], facilitated large-scale ascertainment of nearly 2000 young lineage-specific insertions. This study provides candidate oligonucleotides for locus-specific PCR assays for over 1350 of these elements. For 314 elements across four taxa with multiple specimens, PCR analyses identified 159 insertion polymorphisms, including 21 grouping and (red-necked species) as sister taxa, with and (gray-necked) being more basal. DNA sequencing identified five novel elements from three different taxa. The datasets reported in this study will assist in species identification and provide a valuable resource for phylogenetics, population genetics and conservation strategies when applied to wild populations.
Publication Source (Journal or Book title)
Storer, J. M., Walker, J. A., Rewerts, L. C., Brown, M. A., Beckstrom, T. O., Herke, S. W., Roos, C., & Batzer, M. A. (2022). Owl Monkey Insertion Polymorphisms and Phylogenetics. Genes, 13 (11) https://doi.org/10.3390/genes13112069