Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease
An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.
Publication Source (Journal or Book title)
Journal of bacteriology
Earl, A. M., Rankin, S. K., Kim, K., Lamendola, O. N., & Battista, J. R. (2002). Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease. Journal of bacteriology, 184 (4), 1003-9. https://doi.org/10.1128/jb.184.4.1003-1009.2002