Title
Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology
Document Type
Article
Publication Date
1-1-1988
Abstract
An alcohol dehydrogenase (ADH) null mutant of Drosophila melanogaster (AdhnLA405) originally recovered following X-ray irradiation of mature sperm (Aaron, 979) is analyzed by Southern blotting, Western blotting, and DNA sequencing. The genetic, immunologic, and nucleic acid sequence data are consistent with the hypothesis that a cross-over event, independent of X-irradiation, between parental chromosomes is responsible for the ADH null phenotype of AdhnLA405. By DNA-sequence analysis we show that molecular cloning of this locus (i.e., propagation in prokaryotic hosts) apparently does not introduce any spurious changes (substitutions, additions, deletions, or rearrangements) within the DNA. © 1988.
Publication Source (Journal or Book title)
Mutation Research Letters
First Page
53
Last Page
56
Recommended Citation
LoMonaco, M., Batzer, M., Tedeschi, B., Chang, S., & Lee, W. (1988). Molecular analysis of Drosophila melanogaster AdhnLA405 confirms reliability of DNA-sequencing methodology. Mutation Research Letters, 207 (2), 53-56. https://doi.org/10.1016/0165-7992(88)90041-3