Phylogenetic analysis of species of three subgenera of coryphaenoides (Teleostei:Macrouridae) by Peptide mapping of homologs of ldh-a4

Raymond R. Wilson, University of South Florida St. Petersburg
Joseph F. Siebenaller, Louisiana State University
Bonnie J. Davis, Louisiana State University


Homologs of muscle-type (A4) lactate dehydrogenase from nine species of Coryphaenoides and one species of Coelorhynchus were compared by tryptic digestion and peptide mapping using reversedphase HPLC. Data on the HPLC profile of peptides from pairwise comparisons were coded as discrete, two state characters and as dissimilarity values. The data were then analysed using Wagner parsimony and leastsquares methods (Fitch-Margoliash), respectively. The four resulting trees agreed in uniting Coryphaenoides (Nematonurus) armatus and Coryphaenoides (Chalinura) leptolepis as a monophyletic group, but did not agree on the arrangement of species within the genus Coryphaenoides, and did not support the present subgeneric arrangement of Coryphaenoides. The result for Coryphaenoides (N.) pectoralis did not support a recent suggestion, based on morphology, that this species be assigned to its own monotypic genus, Albatrossia. Peptide mapping as a technique for systematic research appears most useful at the intergeneric level of divergence. © 1991.